An experimental study of the harmful effects of diffuse day-light was already
reported in a previous thesis.
This study was conducted to confirm the harmful effect of artificial lights to B.C.G.
viability during production and handing. A comparative study on protection of B.C.G.
viable counts was conducted on brown and clear ampoules. under ultra red ray. ultra
violet ray, fluorescense electric bulb ray, tangsten electric bulb ray, mercury lamp ray,
candle flame, petroleum lamp flame, alcohol lamp flame, liquid petroleum gas flame
alcohol lamp flame, liquid petroleum gas flame and LPG.
plus oxygen flames, In this experiment 31-42 million viable units of B.C.G. vaccine 1
㎎ per c.c. were used.
The results under artificial-light on Korean liquid B.C.G. vaccine viability obtained
were as follows;
1. Under 50 ㎝ distance, exposed to 100v-100W bulb of tangsten electric ray, no effect
was found on viable units in 5 hours and no significant difference was observed
between brown and clear ampoules.
2. Under exposure of 120V-30W bulb fluorescense electric ray, effect on the viable
counts was same as tangsten bulb ray.
3. At 50 ㎝ distance, exposed to 125 V-250 W bulb of ultra red ray, the viable units
were reduced by 50% in 2 hours and no living bacteria were found after exposing for
3 to 4 hours. No significant difference was observed the brown and clear ampoules.
4. Under 50 ㎝ distance, exposed to 100 V--19 W bulb of ultra violet ray, tile viable
units wire reduced by 20% in 4 hours and no living bacteria were found after exposure
for 5 hours, There was sudden red uction after 4 hours. No significant difference was
confirmed between brown and clear ampoules.
5. At 50 ㎝ distance, exposed to 115 V-l00 W bulb of mercury lamp ray, no effect on
the viable units of B.C.G. vaccine were observed.
6. When given on 6 hours exposure to tile 2.0 8.0 ㎝ flame.
size of candle flame, petroleum flame, alcohol lamp flame, liquid petroleum gas flame
and LPG plus oxygen flames, at distance of 15 ㎝, no effect on the viable flames, at
distance of 15 ㎝, no effect on the viable units were observed under all circumstances.
No significant difference was observed between brown and clear ampoules.
|